Relevant Intravenous Injection in Mice: A Comprehensive Guide

The intravenous injection technique is a crucial procedure in murine research. This blog post aims to provide a comprehensive guide on how to perform intravenous injections in mice to ensure accurate drug delivery and minimal harm to the animals. Whether you are conducting studies in pharmacology, oncology, or any other field that requires precise intravenous administration, understanding the proper technique is paramount.

Why Intravenous Injection in Mice?

Administering drugs intravenously in mice offers several advantages over other routes of administration. It allows for rapid and precise delivery of substances directly into the bloodstream, ensuring a faster onset of action and accurate dosing. Additionally, intravenous injection provides a more controlled environment for studying drug pharmacokinetics, distribution, and clearance in preclinical research.

Preparation and Equipment

Before commencing intravenous injections, you must gather the necessary equipment:

  • Sterile syringes and needles suitable for mice
  • Appropriate anesthetic agents
  • Disinfectant solution
  • Gloves
  • Alcohol swabs
  • Bandages or cotton swabs

Ensure all instruments are sterile and in good condition to prevent contamination and minimize any potential complications during the injection process.


1. Prepare the working area:

Clean the laboratory bench with a suitable disinfectant to maintain a sterile environment. Wear gloves throughout the procedure to prevent contamination and to protect yourself from hazardous substances.

2. Anesthetize the mouse:

Administer an appropriate dose of anesthetic agent according to the approved protocol to ensure the well-being and comfort of the mouse during the injection.

3. Restrain the mouse:

Gently restrain the mouse in an appropriate manner to minimize movement during the procedure. A specialized restraining device can be used to keep the mouse in place comfortably.

4. Prepare the injection site:

Disinfect the injection site, typically the tail vein, with an alcohol swab or another suitable antiseptic. Allow the area to dry before proceeding further.

5. Assemble the syringe:

Attach the needle to the syringe, ensuring it is secure and properly aligned. Draw the correct dose of the substance into the syringe based on the experimental requirements.

6. Administer the injection:

Hold the syringe at a slight angle and insert the needle into the vein at the site previously disinfected. Slowly inject the substance while closely monitoring the mouse’s reaction.

7. Withdraw the needle:

Once the injection is complete, withdraw the needle carefully to avoid any tissue damage or bleeding. Apply gentle pressure to the injection site with a cotton swab or bandage to prevent blood leakage.

Post-Injection Care

Post-injection care is crucial to ensure the well-being of mice and minimize complications:

  • Monitor the mouse closely for any adverse effects or signs of distress.
  • Provide appropriate post-injection pain relief if required.
  • Dispose of used needles and syringes in a designated sharps container to maintain safety.
  • Clean the work area and disinfect any contaminated surfaces.


This comprehensive guide has provided an overview of the intravenous injection technique in mice. By following the proper steps and considering the well-being of the animals, accurate and reliable data can be obtained in various preclinical studies. Always remember to adhere to the approved protocols and seek guidance from a trained professional when performing intravenous injections in mice.

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